2009

Leskinen, S. D.,  E. A. Kearns and D. V. Lim. 2009. Dead-end hollow fiber ultrafiltration with the automated concentration system for simultaneous recovery of Bacillus atrophaeus spores and MS2 bacteriophage directly from chloraminated tap water. Abstr. Ann. Meeting ASM, Philadelphia, PA.

Hunter, D. M.,  S. D. Leskinen and D. V. Lim. 2009. Detection of waterborne pathogens in concentrated recreational water using an ATP bioluminescence immunoassay coupled to PCR. Abstr. Ann. Meeting ASM, Philadelphia, PA.

Kearns, E. A.,  S. Leskinen and D. V. Lim. 2009. Characterization of an improved filter for recovery of microorganisms from water using an automated concentration system. Abstr. Ann. Meeting ASM, Philadelphia, PA.

Schlemmer, S. M., S. Magaña, and D. V. Lim. 2009. Method to concentrate and detect: Automated concentration system and lateral flow immunoassays. Abstr. Ann. Meeting ASM, Philadelphia, PA.

Kearns, E.A., S. D. Leskinen, J. M. Stroot, S. Magaña, S. Schlemmer, D. Hunter and D. V. Lim. 2009. Developing and Optimizing Technologies for the Detection of Microorganisms. 9th Ann. Meet. Consortium Biodefense Res., Tampa, FL.

Leskinen, S. D., E. A. Kearns and D. V. Lim. 2009. Rapid Detection of Pathogens in Water: The Need for Concentration. 9th Ann. Meet. Consortium Biodefense Res., Tampa, FL.

Lim, D. V., E. A. Kearns, S. D. Leskinen, J. M. Stroot, S. Magaña, S. Schelmmer, and D. Hunter. 2009. Sample Preparation and Assay Development for Rapid Food Surveillance. DARPA SAFE WEST, San Francisco, CA.

2008

Thompson, R. and D. V. Lim. 2008. Bacteriophage P68 Protein 17 as a capture and detection molecule for Staphylococcus aureus in biosensor assays. Abstr. Ann. Meeting ASM. Boston, MA

Schlemmer, S. M., S. D. Leskinen, and D. V. Lim. 2008. Indirect detection of gram negative bacteria by polymyxin B sulfate with a fiber optic biosensor. Abstr. Ann. Meeting ASM. Boston, MA

Leskinen, S. D. and D.V. Lim. 2008. Rapid assessment of enterococci in southern California recreational water by ultrafiltration concentration coupled to biosensor detection. Abstr. Ann. Meeting ASM. Boston, MA

Nyquist-Battie, C., D. Lund, J.E. Chu, E. Miller, and D.V. Lim. 2008. Detection of Vibrio cholerae using antisera produced by immunization with a synthetic peptide containing the sequence of a surface-exposed region of toxin-coregulated pilus protein A (TcpA). Abstr. Ann. Meeting ASM. Boston, MA

Hunter, D. M. and D. V. Lim. 2008. Rapid detection and identification of bacterial pathogens using an ATP bioluminescence immunoassay. Abstr. Ann. Meeting ASM. Boston, MA

Hunter, D. M., and D. V. Lim. 2008. Detection of Escherichia coli O157:H7 in 10-gram ground meat samples using an evanescent wave figer-optic biosensor. SE Branch Am. Soc. Microbiol. Ann.Meeting. Jacksonville, FL.

2007

Nyquist-Battie, C., A. Watsky, D. Lund, K. Clark, and D.V. Lim. 2007. Antibody-based Assays for detection of Enterotoxigenic (ETEC) and shiga-toxin (STEC) Escherichia coli. 5th Ann. ASM Biodefense Emerg. Dis. Res. Meeting. Washington, D.C.

Kearns, E.A., S. Magaña, and D.V. Lim. 2007. Concentration of Escherichia coli 0157:H7 from drinking water using an automated concentration system. Abstr. Ann. Meeting ASM. Toronto, CAN.

Kramer, M.F., U. Bläsi, and D.V. Lim. 2007. Use of phage P68 virion associated protein 17 for detection of whole cells of Staphylococcus aureus using biosensor assays. Abstr. Ann. Meeting ASM. Toronto, CAN.

Leskinen, S.D., V.J. Harwood, and D.V. Lim. 2007. Near real-time detection of enterococci in recreational coastal waters by the Rec DEC-RAPTOR method. Abstr. Ann. Meeting ASM. Toronto, CAN.

Magaña, S., M.F. Kramer, and D.V. Lim. 2007. Cross-reactivity of Escherichia coli O157:H7 antibody with wastewater background flora. Abstr. Ann. Meeting ASM. Toronto, CAN.

Nyquist-Battie, C., E. Miller, D. Lund, and D. V. Lim. 2007. Production of polyclonal antibodies for the detection of Salmonella enterica serovars Typhimurium and Enteritidis using bacterial strains lacking the O antigen of the lipopolysaccharide. Abstr. Ann. Meeting ASM. Toronto, CAN.

Schlemmer, S.M., M.F. Kramer, C. Nyquist-Battie, and D.V. Lim. 2007. Capture efficiency of gram negative bacteria by polymyxin B sulfate is increased by detergent in biosensor assays. Abstr. Ann. Meeting ASM. Toronto, CAN.

Kearns, E.A., S. Magaña, and D.V. Lim. 2007. Concentration of Escherichia coli 0157:H7 from drinking water using an automated concentration system. SE Branch Am. Soc. Microbiol. Ann.Meeting. Auburn, AL.

Leskinen, S.D. and D.V. Lim. 2007. Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of Southern California coastal marine waters. SE Branch Am. Soc. Microbiol. Ann.Meeting. Auburn, AL.

Schlemmer, S.M., S.D. Leskinen, M.F. Kramer, C. Nyquist-Battie, and D.V. Lim. 2007. Boiling cells in detergent increases capture of Escherichia coli O157:H7 cells by polymyxin B sulfate. SE Branch Am. Soc. Microbiol. Ann.Meeting. Auburn, AL.

Kearns, E.A., S. Magaña, and D.V. Lim. 2007. Rapid detection of pathogens in water – are we there yet? Florida Section, AWWA Fall Meeting. Orlando, FL.

Lim, D.V. 2007. Biosensors, homeland security and defense. Florida Research Consortium 4th Annual Tech Transfer Conference. Miami, FL.

2006

Denton, K.A. and D.V. Lim. 2006. Rapid detection of Mycobacterium tuberculosis using a fiber optic biosensor. Abstr. Ann. Meeting ASM.

Kearns, E.A., S. Magaña, T. Postlethwaite, R. Sorrells, and D.V. Lim. 2006. Development of an automated on-line water monitor: field test results. Abstr. Ann. Meeting ASM.

Kramer,.M.F., U. Bläsi, and D.V. Lim. 2006. Use of phage P68 virion associated Protein 17 for detection of whole cells of Staphylococcus aureus using enzyme-linked assays. Abstr. Ann. Meeting ASM.

Mercado, Q.J., M.F. Kramer, and D.V. Lim. 2006. Use of polymyxin B for capture of gram negative bacteria in rapid detection of gram-negative bacteria using a fiber-optic biosensor. Abstr. Ann. Meeting ASM.

Shehane, S.D., M.T. Trindade, D. Wingfield, and D.V. Lim. 2006. Time required for detection of Enterococcus spp. in recreational coastal waters - Analyte 2000 biosensor assay versus EPA Method 1600. Abstr. Ann. Meeting ASM.

Nyquist-Battie, C., D. Lund, and D.V. Lim. 2006. Expression of single-chain variable-fragment (scFv) recombinant antibodies against cholera toxin in an insect cell line. Abstr. Ann. Meeting ASM.

Nyquist-Battie, C., J-P. S. Soberano, L. Freeman, D. Lund, and D. V. Lim. 2006. Suitability of polyclonal antibodies to the rough mutant Escherichia coli J5 for immunoassay-based detection of the fecal indicator, Eshcerichia coli. Abstr. Ann. Meeting ASM.

Nyquist-Battie, C, J-P.S. Soberano, L. Freeman, K. Leckband, D. Lund, and D.V. Lim. 2006. Screening commercial polyclonal antibodies against the Escherichia coli reference collection (ECOR) for use in portable, evanescent wave, fiber-optic biosensors. Am. Soc. Microbiol. Biodefense Meet., Washington, DC.

Kramer, M.F. and D.V. Lim. 2006. Affinity-based binding molecules in biosensor assay development. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Kearns, E.A., S.D. Leskinen, and D.V. Lim. 2006. You need to concentrate: Detection of microorganisms in water. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Simpson, J.M. and D.V. Lim. 2006. Novel Biosensor Applications Using Fluorescent Technologies. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Kearns, E.A., S.Magaña, T. Postlethwaite, R. Sorrells, and D.V. Lim. 2006. Results from field tests of an automated concentration-detection system for water monitoring. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Mercado, Q,.J. M.F. Kramer, and D.V. Lim. 2006. Use of polymyxin B for capture of Gram negative bacteria in rapid detection of Gram-negative bacteria using a fiber-optic biosensor. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Nyquist-Battie, C., J-P.S. Soberano, L. Freeman, D. Lund, and D.V. Lim. 2006. Antibodies for the detection of pathogenic and non-pathogenic Escherichia coli in water and food. Ann. Meeting Consortium Biodefense Research., Clearwater, FL.

Kearns, E.A., S.L. Leskinen, S.D. and D.V. Lim. 2006. You need to concentrate: Detection of microorganisms in water. SE Branch Am. Soc. Microbiol. Ann. Meeting, Kennesaw, GA.

Simpson, J.M. and D.V. Lim. 2006. Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. SE Branch Am. Soc. Microbiol. Ann. Meeting, Kennesaw, GA.

 Nyquist-Battie, C., A. Watsky, D. Lund, K. Clark, and D.V. Lim. 2006. Identification of Escherichia coli virotypes using antibody-based assays. SE Branch Am. Soc. Microbiol. Ann. Meeting, Kennesaw, GA.

Lim, D.V., S.D. Leskinen, E.A. Kearns, and S. Magaña. 2006. Rapid biosensor detection of waterborne pathogens. BioFlorida/Florida Marine Biotech Summit, Gainesville, FL.

Lim, D.V. 2006. Rapid Biosensor Detection of Pathogens, Florida SBIR/STTR Conference, Tampa, FL.

2005

Bedenbaugh, C., M.F. Kramer, and D.V. Lim. 2005. A ganglioside-based assay for the detection of cholera toxin in oyster homogenate utilizing an evanescent wave fiber-optic biosensor. Abstr. Ann. Meeting ASM.

Kearns, E.A., S. Magaña, B. Dodge, A. Levine, R. Sorrells, and D.V. Lim. 2005. Development of a semi-dead end filtration system for concentration of bacterial spores from drinking water. Abstr. Ann. Meeting ASM.

Kramer, M.F. and D.V. Lim. 2005. Use of LPS-binding peptides for capture of gram negative bacteria in rapid detection of gram-negative bacteria using a fiber-optic biosensor. Abstr. Ann. Meeting ASM.

Magaña, S., E.A. Kearns, D.V. Lim, A.K. Bailey, M.V. Tesone, and T.A. Postlethwaite. 2005. Development of rapid biosensor assays to detect B. globigii and E. coli O157:H7 using an automated array biosensor. Abstr. Ann. Meeting ASM.

Simpson, J.M. and D.V. Lim. 2005. Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. Abstr. Ann. Meeting ASM.

Trindade, M.T., M.F. Kramer, and D.V. Lim. 2005. An evanescent wave fiber optic biosensor for the detection of fecal enterococci in beach water. Abstr. Ann. Meeting ASM.

Lian, W., D.V. Lim, and S. Jin. 2005. Sensitive detection of pathogens and toxins using fluorescent nanoparticles in a microarray format. Abstr. Ann. Meeting ASM.

Lund, D., C. Nyquist-Battie, M.F. Kramer, and D.V. Lim. 2005. Isolation of anti-cholera toxin single chain Fv (scFv) antibody fragments from Tomlinson libraries using phage display followed by expression in Escherichia coli HB2151 cells. Abstr. Ann. Meeting ASM.

Freeman, L., C. Nyquist-Battie, D. Lund, and D.V. Lim. 2005. Effect of chlorine disinfectant on antibody-based detection of Escherichia coli O157:H7. Abstr. Ann. Meeting ASM.

2004

Bedenbaugh, C.M, M.F. Kramer, and D.V. Lim. 2004. A ganglioside-based assay for the detection of cholera toxin in oyster homogenate utilizing an evanescent wave fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 501.

Donaldson, K.A., N.L. Look, T.B. Tims, and D.V. Lim. 2004. The electronic TasteChip, a flow-through, microchip multi-analyte detection system. A ganglioside-based assay for the detection of cholera toxin in oyster homogenate utilizing an evanescent wave fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 661.

Kearns, E.A., A. Bissing, T.B. Tims, S. Dickey, and D.V. Lim. 2004. Concentration of B. subtilis var. niger spores from water followed by detection using a fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 592.

Kramer, M.F., N.L. Look, and D.V. Lim. 2004. Anti-Cryptosporidium oocyst polyclonal antibody utilized in immunoassays detects soluble antigen of Cryptosporidium parvum oocysts. Abstr. Ann. Meeting ASM, p. 551.

Look, N.L., M.F. Kramer, V.A. Luna, and D.V. Lim. 2004. Use of Bacillus thuringiensis spore coat proteins for antibody production and rapid biosensor immunoassay development. Abstr. Ann. Meeting ASM, p. 660.

Nyquist-Battie, C., L. Freeman, D. Lund, and D.V. Lim. 2004. Improvement of sandwich enzyme-linked immunoabsorbent assay detection of Escherichia coli O157:H7 in apple juice by the addition of polyvinylpyrrolidone. Abstr. Ann. Meeting ASM, p. 482.

Trindade, M.T., M.F. Kramer, and D.V. Lim. 2004. An evanescent wave fiber optic biosensor for the detection of fecal enterococci in beach water. Abstr. Ann. Meeting ASM, p. 548.

2003

Donaldson, K.A., M.F. Kramer, and D.V. Lim. 2003. Detection of Vaccinia virus, a surrogate of the smallpox virus, from seeded throat culture swabs using a fiber optic biosensor assay. Abstr. Ann. Meeting ASM, p. 182.

Nyquist-Battie, C., D. Lund, K.A. Kearns, and D.V. Lim. 2003. F(ab')2 antibody fragments used as secondary antibodies improve detection of Escherichia coli O157:H7 in sandwich and non-sandwich enzyme-linked immunoassays (ELISAs) using commercially-available primary antibodies. Abstr. Ann. Meeting ASM, p. 498.

Kramer, M.F., P.A.Gulig, and D.V. Lim. 2003. A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seeds. Abstr. Ann. Meeting ASM, p. 500.

Trindade, T., M.F. Kramer, and D.V. Lim. 2003. Antibody-based detection of microbial pathogens and indicator organisms in marine water utilizing an evanescent wave fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 550.

Kearns, E.A., T.B. Tims, and D.V. Lim. 2003. The effect of chlorine, chloramines and chlorine neutralization on detection of Escherichia coli O157:H7 with an antibody-based fiber optic biosensor assay. Abstr. Ann. Meeting ASM, p. 604.

Bedenbaugh, C.M., M.F. Kramer, and D.V. Lim. 2003. A ganglioside-based assay for the detection of cholera toxin utilizing an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 650.

Look, N.I., M.F. Kramer, and D.V. Lim. 2003. Extraction and detection of Bacillus anthracis proteins for antigenic recognition of the spores. Abstr. Ann. Meeting ASM, p. 651.

2002

Bedenbaugh, C.M., M.F. Kramer, and D.V. Lim. 2002. A ganglioside-based assay for the identification of cholera toxin utilizing an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 37.

Tims, T. and D.V. Lim. 2002. Recovery of Bacillus globigii spores from fiber optic waveguides after rapid identification with an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 105.

Kramer, M.F., T.B. Tims, D.R. DeMarco, and D.V. Lim. 2002. Recovery of Escherichia coli O157:H7 from fiber optic waveguides used for rapid detection. Abstr. Ann. Meeting ASM, p. 369.

DeMarco, D.R., T.B. Tims, and D.V. Lim. 2002. Biosensor detection and recovery of Escherichia coli O157:H7 from human fecal samples. Abstr. Ann. Meeting ASM, p.134.

de Groh, E.D., M.A. Psiaki, V.J. Harwood, and D.V. Lim. 2002. Sensitivity and specificity of Escherichia coli O157:H7 detection in untreated wastewater using an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p.432.

Varkey, S., A.C. Cannons, and D.V. Lim. 2002. Enhanced detection of DNA fragments with an evanescent wave biosensor using a signal amplification system. Abstr. Ann. Meeting ASM, p.145.

Trindade, T., M.F. Kramer, and D.V. Lim. 2002. Antibody-based detection of fecal enterococci utilizing an evanescent wave fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 437.

Dickey, S.S.,M.F. Kramer, and D.V. Lim. 2002. Recovery of Staphylococcus aureus on surfaces using a swab-rinse technique vs. immersion technique. Abstr. Ann. Meeting ASM, p. 259.

Delahoya, N.C., M.F. Kramer, J.E. Whitlock, V.J. Harwood, and D.V. Lim. 2002. Survey of Enterococcus species from various sources using API 20 Strep and PCR methods for species identification. Abstr. Ann. Meeting ASM, p. 389.

2001

Kryder, C.D. and D.V. Lim. 2001. Detection of Cy5-labeled oligonucleotides with an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 156.

DeMarco, D.R. and D.V. Lim. 2001. Rapid detection of Escherichia coli O157:H7 in 10 and 25 gram ground beef samples using an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 573.

Tims, R., S.S. Dickey, and D.V. Lim. 2001. Detection of low levels of Listeria monocytogenes within 12-16 hours by an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 575.

2000

DeMarco, D.R. and D.V. Lim. 2000. Direct detection E. coli O157:H7 in apple juice and apple cider using a portable, evanescent wave fiber optic biosensor. Abstr. Ann. Meeting ASM, p. 512.

Tims, T.B., D.V. Lim, and D.R. DeMarco. 2000. Rapid detection of Listeria monocytogenes with an evanescent wave biosensor. Abstr. Ann. Meeting ASM, p. 518.

Ray, C.D. and D.V. Lim. 2000. Erythromycin determinants ErmB and MefA/E in group B Streptococcus. Abstr. Ann. Meeting ASM, p. 15.

1999

1999 DeMarco, D.R. and D.V. Lim. 1999. A portable evanescent wave fiber optic biosensor for the rapid detection of Escherichia coli O157:H7 in ground beef. Abstr. Ann. Meeting ASM, p. 513.

Ray, C.D. and D.V. Lim. 1999. Antibiotic resistance in group B Streptococcus. Abstr. Ann. Meeting ASM, p. 160.

Morrow, M.R., R. Widen, and D.V. Lim. 1999. Detection of group B Streptococcus using flow cytometry after capturing with paramagnetic beads. Abstr. Ann. Meeting ASM, p. 196.

Coakley, M.B. and D.V. Lim. 1999. Purification of a collagenase from group B Streptococcus. Abstr. Ann. Meeting ASM, p. 241.

LeHoullier, L.. and D.V. Lim. 1999. Induction of a collagenase in group B Streptococcus. Abstr. Ann. Meeting ASM, p. 241.